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Comparison of different cell disruption techniques
using a Soniprep 150 sonicator and nano deBEE homogeniser
October 2009
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| Drug development services company Almac have recently completed a comparison of high-pressure homogenisation
and sonication in cell disruption, using the deBEE "nano" system.
Cell pastes were treated to different homogenisation treatments using the nano deBEE high-pressure and a Soniprep 150
sonicator. Once disrupted the cells were spun in a centrifuge to separate the cell pellet from supernatant and tested for
protein concentration using gel electrophoresis. The experiment showed that two passes of cells throughe nano deBEE
homogeniser was the more efficient method of cell disruption.
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Above: cell pellets and
supernatants tested by
gel elecrophoresis;
below, the nano deBEE
homogeniser
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The success of high pressure homogenisation was tested as sonication, although widely used in the laboratory, is not suitable
for scale-up. The patented deBEE homogenising cell technology is designed to scale linearly from volumes of around 150ml/minute in
the nano system, to 30litres/minute or more in their production systems.
Almac offers a comprehensive range of integrated drug discovery services for the pharma and biotech industries.
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